Data/hora: 01/06/2024 12:30:16

Provedor de dados: | 65
País: | Brazil
Título: | Brazilian ground beef authentication by multiplex polymerase chain reaction
Autores: | Oliveira,Andrey Carlos do Sacramento de; Pedroso,Silvia Cristina da Silva; Cardilli,Diogo José; Leite,Fábio Pereira Leivas; Ferreira,Gabrielle Virgínia Lopes; Silva,Andréia Silva da; Roos,Talita Bandeira; Moraes,Carina Martins de; Sousa,Roberta Sales; Monteiro,Roseli do Socorro Dias.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Species identification; Bos taurus; Bubalusbubalis; DNA; Adulteration.
Resumo: | ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20160574
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.2 2018
Fonte: | info:eu-repo/semantics/openAccess