Data/hora: 06/06/2024 05:43:20

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Microshoots encapsulation and plant conversion of Musa sp. cv. 'Grand Naine'	
Autores: 	Sandoval-Yugar,Edgar Wilfredo; Dal Vesco,Lírio Luiz; Steinmacher,Douglas André; Stolf,Elaine Cristina; Guerra,Miguel Pedro.	
Data: 	2009-07-01	
Ano: 	2009	
Palavras-chave: 	Artificial seeds; Banana; In vitro propagation; Pharmaceutical capsules.	
Resumo: 	Synthetic seed technologies are useful tools for the field delivery of in vitro derived plantlets. In the present study, different encapsulation procedures and their efficacy in the plantlet regeneration using microshoots of banana cv. 'Grand Naine' were evaluated. Two encapsulation systems were evaluated: i) single encapsulation in beads or droplet hardening method; and ii) double layer or hollow beads. The use of different compounds to enhance the capsule conservation and the conversion to plantlets was also evaluated. The conversion capacity was assessed in vitro on water-agar culture medium or in ex vitro conditions with Gerbox® boxes. The single encapsulation system showed 80% conversion. The capsules with MS saline formulation treated with 100mM KNO3 showed 76% conversion. Capsules with 1.5g L-1 activated charcoal, and 0.5g L-1 benomyl sucrose-free capsules showed 75% conversion. The encapsulated and non-encapsulated microshoots exhibited 100% germination in response to MS culture medium, and polyethylene glycol after 10 days of storage at 4°C. Sucrose-free capsules showed significantly higher germination (83.3%) than those sucrose-enriched capsules (56.7%). The ex vitro conversion of encapsulated microshoots was 20% in the Gerbox™. These results indicate the feasibility using synthetic seeds in the large-scale micropropagation of banana cv. 'Grand Naine'.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000400007	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/S0103-84782009005000024	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.39 n.4 2009	
Fonte: 	info:eu-repo/semantics/openAccess