Data/hora: 13/06/2024 14:33:55

Provedor de dados: | 65
País: | Brazil
Título: | In vitro shoot regeneration of boldo from leaf explants
Autores: | Mota,Monalize Salete; Bandeira,Juliana de Magalhães; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio.
Data: | 2010-10-01
Ano: | 2010
Palavras-chave: | Plectranthus neochilus; Organogenesis; Plant growth regulators.
Resumo: | A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001000026
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782010005000173
Formato: | text/html
Fonte: | Ciência Rural  v.40 n.10 2010
Fonte: | info:eu-repo/semantics/openAccess