Data/hora: 06/06/2024 06:28:35

Provedor de dados: | 65
País: | Brazil
Título: | Molecular evidence of early vertical transmission of Leishmania ( Leishmania ) infantum in a dog
Autores: | Oliveira,Vinícius Vasconcelos Gomes de; Ramos,Rafael Antonio Nascimento; Ramos,Carlos Alberto do Nascimento; Guerra,Neurisvan Ramos; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Leishmaniasis; Dog; Vertical infection; Embryo.
Resumo: | ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon  Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The gestational age was estimated to be 23±1 day. Through qPCR, the presence of L. infantum DNA was detected in all the samples analyzed (n=7), including the embryo, conversely through cPCR, only four samples (vagina, cervix, uterine body and embryo) were positive. This study demonstrated that transmission of L. infantum from a pregnant dog to the embryo might occur in the early days of pregnancy. In conclusion, this is the first report showing L. infantum infecting a canine embryo.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20160553
Formato: | text/html
Fonte: | Ciência Rural  v.47 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess