Data/hora: 11/06/2024 03:45:38

Provedor de dados: | 65
País: | Brazil
Título: | Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil
Autores: | Brilhante,Andreia Fernandes; Dorval,Maria Elizabeth Cavalheiros; Galati,Eunice Aparecida Bianchi; Castro,Vivianne de Oliveira Landgraf de; Rocca,Maria Elizabeth Ghizzi; Facco,Gilberto Gonçalves; Souza,Celeste da Silva Freitas de; Nunes,Vânia Lúcia Brandão.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Domestic dogs; Serodiagnosis; Ecotourism.
Resumo: | ABSTRACT: The study aimed to investigate Leishmania infection in a population of dogs (92 animals) in a fishing area of Bonito Municipality, and evaluate comparatively the serological methods used, immunoenzymatic assay (ELISA), indirect immunofluorescence antibody test (IFAT), and rapid Dual Path-Platform test (DPP®). Blood and aspirate of bone marrow samples were used and parasitological investigation was also performed, such as parasite isolation in Neal, Novy, Nicolle (NNN) medium culture, Woo technique, Giemsa stained smears and specific identification by polymerase chain reaction (PCR). IFAT revealed 56/92 seropositive, the ELISA 8/92 and the DPP®41/92. Regarding the agreement between the serological tests by the Kappa index, there was a slight agreement between ELISA/IFAT and ELISA/DPP®, and fair agreement in IFAT/DPP®. The NNN culture was positive in three out of 21 dogs, and identified by PCR as Leishmania infantum chagasi. No samples were positive by the Woo technique. Our results showed low agreements between the serological tests recommended by the Brazilian Ministry of Heath, and it is necessary to associate other diagnostic techniques, such as parasitological tests and PCR, to increase the sensitivity for canine visceral leishmaniosis diagnosis, mainly regarding asymptomatic dogs in endemic areas.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300501
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170332
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess