Data/hora: 11/06/2024 09:54:25

Provedor de dados: | 65
País: | Brazil
Título: | A new, harmless, high-throughput endosperm-based DNA extraction method for wheat
Autores: | Ma,Zhihui; Wang,Yuquan; Wei,Wenhui; Ru,Zhengang.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Wheat; Triticum aestivum; SSR; Segregation population.
Resumo: | ABSTRACT:  In this study, a non-destructive, high-throughput, endosperm-based DNA extraction method was developed. To verify the non-destructive nature of this method, a germination test was performed on 288 seeds after sampling their endosperm, which gave a seedling emergence rate that was higher (97.6%) than that of the control group (92%). To confirm the feasibility of the new method, DNA was extracted from plants of a BC1F2 population by two different methods, namely, from endosperm using our rapid, high-throughput method (ER-DNA) and from young leaves emerging from the same sampled seed using the CTAB method (LC-DNA). The ER-DNA was undetectable by agarose gel electrophoresis, but was found to be an adequate replacement for LC-DNA for the amplification and detection of simple sequence repeats (SSRs). Further analysis revealed that ER-DNA was generally suitable for the generation of specific 500-750-bp fragments, but not for the amplification of 1,000-2,000-bp fragments. Our rapid, high-throughput method therefore has no deleterious effects on wheat seeds and yields DNA for SSR genotyping that is a suitable alternative to traditionally obtained DNA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900200
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170936
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.9 2019
Fonte: | info:eu-repo/semantics/openAccess