Data/hora: 10/06/2024 18:22:18

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Genetic transformation of the Brazilian BR 451 maize variety by the Agrobacterium tumefaciens method	
Autores: 	Silva,Marilia Rodrigues de; Didoné,Dielli Aparecida; Ceccon,Cássia Canzi; Almeida,Vinícius de Oliveira; Grando,Magali Ferrari.	
Data: 	2017-11-01	
Ano: 	2017	
Palavras-chave: 	In vitro regeneration; Temperature stress; EHA 101; PTF102; Zea mays.	
Resumo: 	ABSTRACT: The asexually gene introduction by genetic engineering has brought enormous possibilities to innovate plant breeding. However, principally because of the low in vitro response, genetic transformation has been restricted to only certain genotypes of agronomically significant species. With the objective of establishing a protocol for genetically transforming the Brazilian BR 451 maize variety through Agrobacterium tumefaciens, it was studied the capacity of plant regeneration in vitro from embryogenic calli cultivated in three regeneration media, each having different growth regulators. It was also evaluated the temperature stress effect on the transformation of the immature embryos with A. tumefaciens EHA 101 containing the plasmid pTF102 with uidA and bar genes. The BR 451 variety embryos and those of the Hi-II hybrid (control) were exposed to three treatments applied as they were being infected with the agrobacteria (a) infection at 25°C; (b) infection at 40°C; (c) pretreatment at 40°C for 5 seconds followed by infection at 25°C. Transformation was determined by uidA gene expression and through the callus resistant to the herbicide Bialaphos® formation. Embryos infected at 40°C showed a higher degree of genetic transformation in the Hi-II, although the same was not noted in BR 451. When growth regulators were added to the culture medium the number of regenerated BR 451 plants showed no increase.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100151	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20141247	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.47 n.11 2017	
Fonte: 	info:eu-repo/semantics/openAccess