Data/hora: 05/06/2024 08:47:55

Provedor de dados: | 65
País: | Brazil
Título: | Biofilm formation by Prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
Autores: | Ely,Valessa Lunkes; Gressler,Letícia Trevisan; Sutili,Fernando Jonas; Ribeiro,Márcio Garcia; Costa,Mateus Matiuzzi da; Vargas,Agueda Castagna de; Botton,Sônia de Avila.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Prototheca zopfii; Dairy cattle bovine mastitis; Intramammary infections; Microplate biofilm assay; Virulence factor.
Resumo: | ABSTRACT:  Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000200451
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180574
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.2 2019
Fonte: | info:eu-repo/semantics/openAccess