Data/hora: 06/06/2024 04:40:13

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing	
Autores: 	Freitas,Loreta Brandão de; Koehler-Santos,Patrícia; Salzano,Francisco Mauro.	
Data: 	2003-06-01	
Ano: 	2003	
Palavras-chave: 	Triticum aestivum L.; Defense proteins; Genetic variability.	
Resumo: 	Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/S0103-84782003000300016	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.33 n.3 2003	
Fonte: 	info:eu-repo/semantics/openAccess