Data/hora: 11/06/2024 12:22:14

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium	
Autores: 	Gavilan,Natália Helena; Furlan,Fernanda Cardoso; Zorz,Alex Zichner; Oliveira,Leandro Silva de; Campos,Wellington Ferreira; Brondani,Gilvano Ebling.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Autoclaving; Cerrado biome; Active chlorine; In vitro cultivation.	
Resumo: 	ABSTRACT:  Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P&lt;0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P&lt;0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900400	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20170581	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.48 n.9 2018	
Fonte: 	info:eu-repo/semantics/openAccess