Data/hora: 06/06/2024 01:33:22

Provedor de dados: | 65
País: | Brazil
Título: | Steroidogenic enzymes mRNA expression profile and steroids production in bovine theca cells cultured in vitro and stimulated with angiotensin II
Autores: | Rigo,Melânia Lazzari; Dau,Andressa Minussi Pereira; Glanzner,Werner Giehl; Martins,Manoel; Zanella,Renato; Rizzetti,Tiele Medianeira; Comim,Fabio Vasconcellos; Gonçalves,Paulo Bayard Dias.
Data: | 2015-04-01
Ano: | 2015
Palavras-chave: | RAS; LH; Steroidogenesis; Ovary.
Resumo: | The main objective of this study was to detect the steroidogenic effects of Ang II in bovine theca cells in vitro. Bovine theca cells were obtained from follicles (larger than 10mm of diameter) collected from a local abattoir and submitted to different treatments in a sequence of experiments. In experiment 1, CYP17A1 mRNA profile was evaluated in LH- (10ng ml-1) and Ang II-treated (0.1µM) theca cells. In experiment 2, a dose-response effect of Ang II (0.001; 0.1 e 10µM) plus insulin (100ng ml-1) and LH (100ng ml-1) was evaluated on steroidogenesis of bovine theca cells. Experiment 3 explored the effects of saralasin (an antagonist of Ang II receptors) on steroid production and steroidogenic enzymes regulation in theca cells. After 24 hours, culture media from experiments 2 and 3 was collected to evaluate testosterone and androstenedione levels by High-Performance Liquid Chromatography. In parallel, mRNA levels of key steroidogenic enzymes (HSD3B2, CYP11A1, CYP17A1) and STAR were assessed by RT-PCR. There was no difference in testosterone and androstenedione production between treated and controls groups, as well as in mRNA levels of the evaluated genes. In conclusion, the results suggest that Ang II does not regulate steroidogenesis in bovine theca cells
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400704
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20240254
Formato: | text/html
Fonte: | Ciência Rural  v.45 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess