Data/hora: 09/06/2024 06:34:44

Provedor de dados: 	69	
País: 	Chile	
Título: 	A preferable approach to clone hLIF cDNA from the genomic DNA	
Autores: 	Cui,Yihong; Zhu,Guangqin; Chen,Qiuju; Wang,Yunfei; Yang,Mingming; Song,Yuxuan; Wang,Jiangang; Cao,Binyun.	
Data: 	2011-05-01	
Ano: 	2011	
Palavras-chave: 	CDNA cloning; HLIF; Oral mucous membrane; Overlap PCR.	
Resumo: 	Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.14 n.3 2011