Data/hora: 14/06/2024 03:09:33

Provedor de dados: 	69	
País: 	Chile	
Título: 	Development of transformation vectors for the production of potentially high oleate transgenic oil palm	
Autores: 	Masani,Mat Yunus Abdul; Parveez,Ghulam Kadir Ahmad.	
Data: 	2008-07-01	
Ano: 	2008	
Palavras-chave: 	Oleate; Transformation vectors; Transgenic oil palm.	
Resumo: 	The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense &amp;#946;-ketoacyl-ACP-synthase II (KASII) and sense &amp;#916;9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.11 n.3 2008