Data/hora: 09/06/2024 08:30:49

Provedor de dados: | 69
País: | Chile
Título: | Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method
Autores: | Pereira Duta,Flávia; Pessôa de França,Francisca; de Almeida Lopes,Léa Maria.
Data: | 2006-07-01
Ano: | 2006
Palavras-chave: | Exopolysaccharides; Experimental design; Response surface method; Rhizobium sp.
Resumo: | The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 &amp;plusmn; 1)&amp;ordm;C] and pH value (7.0 &amp;plusmn; 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 2³ factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded Y P/S (g/g) = 0.35.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.9 n.4 2006