Data/hora: 09/06/2024 12:35:46

Provedor de dados: 	69	
País: 	Chile	
Título: 	Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method	
Autores: 	Pereira Duta,Flávia; Pessôa de França,Francisca; de Almeida Lopes,Léa Maria.	
Data: 	2006-07-01	
Ano: 	2006	
Palavras-chave: 	Exopolysaccharides; Experimental design; Response surface method; Rhizobium sp.	
Resumo: 	The combined effects of the processing parameters for exopolysaccharides production by Rhizobium sp. was studied using the experimental design and response surface methodology. The experiments were carried out using a fermenter with 20 L capacity, as the reactor. All processing parameters were online monitored. The temperature [(30 &amp;plusmn; 1)&amp;ordm;C] and pH value (7.0 &amp;plusmn; 0.1) were kept constant throughout the experimental time. As statistical tools, a complete 2³ factorial planning with central point and response surface were used to study the interactions among three relevant variables of the fermentation process: calcium carbonate concentration, aeration and agitation. The processing parameters setup for reaching a maximum response for exopolysaccharides production was obtained when applying the highest values for calcium carbonate concentration (1.1 g/L), aeration (1.3 vvm) and agitation (800 rpm). In addition, the combination of these optimum processing parameters yielded Y P/S (g/g) = 0.35.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400007	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.9 n.4 2006