Data/hora: 01/06/2024 08:43:07

Provedor de dados: | 69
País: | Chile
Título: | Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain
Autores: | Zhu,Houze; Jiang,Keji; Zhang,Fengying; Zhang,Dan; Pi,Yan; Ma,Lingbo.
Data: | 2012-03-01
Ano: | 2012
Palavras-chave: | Optic stalk; Potassium acetate; RNA isolation; Scylla paramamosain.
Resumo: | An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified protocol and three conventional methods were also employed to confirm the quality of RNA. This improved method would be helpful in facilitating molecular research of crabs involving RNA from the optic stalk.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200005
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.15 n.2 2012