Data/hora: 16/06/2024 09:26:09

Provedor de dados: 	69	
País: 	Chile	
Título: 	Universal protocol for generating 100bp size standard for endless usage	
Autores: 	Natarajan,Chandrasekhar; Vijayan,Neetha N; Vaidyan,Linda Koshy; Mathew,Anila; Srinivas,Lekshmy; Banerjee,Moinak.	
Data: 	2008-04-01	
Ano: 	2008	
Palavras-chave: 	100bp ladder; Cost effective; DNA size standards; Endless usage.	
Resumo: 	Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.11 n.2 2008