Data/hora: 09/06/2024 14:33:03

Provedor de dados: | 69
País: | Chile
Título: | Ethanol mediated enhancement in bacterial transformation
Autores: | Sharma,Arun Dev; Singh,Jaspreet; Gill,Prabhjot Kaur.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Bacteria; Ethanol; Transformation.
Resumo: | In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.10 n.1 2007