Data/hora: 09/06/2024 01:17:01

Provedor de dados: 	69	
País: 	Chile	
Título: 	Ethanol mediated enhancement in bacterial transformation	
Autores: 	Sharma,Arun Dev; Singh,Jaspreet; Gill,Prabhjot Kaur.	
Data: 	2007-01-01	
Ano: 	2007	
Palavras-chave: 	Bacteria; Ethanol; Transformation.	
Resumo: 	In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.10 n.1 2007