Data/hora: 05/06/2024 16:09:37

Provedor de dados: | 69
País: | Chile
Título: | Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Autores: | Caballero Valdés,Eduardo; Wilson Soto,Lorena; Aroca Arcaya,Germán.
Data: | 2011-05-01
Ano: | 2011
Palavras-chave: | Albumin; Dextran aldehyde; Glutaraldehyde; Immobilisation; Lipase.
Resumo: | The preparation of cross-linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100-200KDa) have a higher hydrolysis activity and particle size (activities between 3186 Â± 21 U/g of CLEA and 4800 Â± 30 U/g of CLEA and particle sizes between 52.6 Â± 18.7 Âµm and 126.2 Â± 53.5 Âµm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 Â± 16 U/g of CLEA and 2874 Â± 20 U/g of CLEA and particle sizes between 21.2 Â± 5.1 Âµm and 83.4 Â± 24.9 Âµm); Thermal stability assays of bioctalysts at 60ÂºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual activity after 50 hrs (maximum residual activity of 46.8% in the CLEA) than CLEAs prepared with glutaraldehyde (maximum residual activity of 70.2% in CLEA). When considering hydrolysis activity, thermal stability and residual activity of CLEAs as a criteria for selecting the best preparation conditions, it has been found that the best condition for CLEAs preparation are to use glutaraldehyde as cross-linking reagent at pH 9.5, at a concentration of 3.5 g/l, and an enzyme/albumin ratio of 15.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300010
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.14 n.3 2011