Data/hora: 06/06/2024 07:21:23

Provedor de dados: 	69	
País: 	Chile	
Título: 	Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum	
Autores: 	Hoenemann,Claudia; Hohe,Annette.	
Data: 	2011-01-01	
Ano: 	2011	
Palavras-chave: 	Gene expression analysis; In vitro propagation; Primer design; Somatic embryogenesis.	
Resumo: 	As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools &amp;#8216;geNorm&amp;#8217; and &amp;#8216;NormFinder&amp;#8217;. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). &amp;#8216;NormFinder&amp;#8217; as well as &amp;#8216;geNorm&amp;#8217; identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes differed depending on the algorithm. According to &amp;#8216;NormFinder&amp;#8217; COG complex component displayed the most stable expression whereas &amp;#8216;geNorm&amp;#8217; indicated V-ATPase G subunit 1 and a putative ABC transporter ATPase to be the most reliable reference genes. Hence, we concluded to use a normalization factor calculated from the four reference genes V-ATPase G subunit 1, ABC transporter ATPase, Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4) and COG complex component for normalization of qRT-PCR in cell cultures of Cyclamen persicum.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100012	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.14 n.1 2011