Data/hora: 11/06/2024 10:37:00

Provedor de dados: | 69
País: | Chile
Título: | Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites
Autores: | Pérez de la Torre,Mariana C.; Escandón,Alejandro S.
Data: | 2006-06-01
Ano: | 2006
Palavras-chave: | ISSR; Ornamentals; Varietal identification.
Resumo: | The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.9 n.3 2006