Data/hora: 07/06/2024 05:42:14

Provedor de dados: | 69
País: | Chile
Título: | Molecular cloning and expression analysis of 12-oxophytodienoate reductase cDNA by wounding in Solanum tuberosum
Autores: | Díaz,Mauricio; Polanco,Victor; Ramírez,Ingrid; Peña-Cortés,Hugo.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | 12-oxophytodienoate reductase; Jasmonic acid; OPDA; Solanum tuberosum; Stress; Wounding.
Resumo: | Jasmonic acid (JA) and 12-oxophytodienoic acid (OPDA) are signal molecules involved in the stress and defense responses in plants. A full-length cDNA clon of OPR3 encoding 12-oxophytodienoate reductase 3, key enzyme involved in the biosynthesis of JA from linolenic acid was obtained from a Solanum tuberosum cDNA library. Sequence analysis showed that OPR3 encoded a polypeptide of 400 amino acids with a predicted molecular mass of 43.9 kDa and pI of 7.72. The deduced amino acid sequence of OPR3 showed high similarities to other 12-oxophytodienoate reductases. A peroxisomal signal sequence indicates OPR3 probable location in peroxisome. Levels of OPR3 mRNA accumulated in potato leaves reaching maximum levels within 1 hr of mechanical wounding. Elevated levels of JA were correlated to expression of the OPR3 gene.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100010
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.15 n.1 2012