Data/hora: 02/06/2024 02:15:47

Provedor de dados: 	69	
País: 	Chile	
Título: 	Heat identification by 17&amp;#946;-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay	
Autores: 	Domènech,Anna; Pich,Sara; Arís,Anna; Plasencia,Carmen; Bach,Alex; Serrano,Alicia.	
Data: 	2011-07-01	
Ano: 	2011	
Palavras-chave: 	Cattle; Estrous; Heat; Hormone; Insemination.	
Resumo: 	Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17&amp;#946;-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17&amp;#946;-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17&amp;#946;-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17&amp;#946;-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17&amp;#946;-estradiol pre-ovulation peak. Conclusions: 17&amp;#946;-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination.	
Tipo: 	Journal article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400010	
Editor: 	Pontificia Universidad Católica de Valparaíso	
Formato: 	text/html	
Fonte: 	Electronic Journal of Biotechnology  v.14 n.4 2011