Data/hora: 13/06/2024 20:00:08

Provedor de dados: | 69
País: | Chile
Título: | Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
Autores: | Pavlov,Maria Soledad; Eissler,Yoanna; Espinoza,Juan Carlos; Garland,David E; Kuznar,Juan.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Digital image analysis; Fluorescence microscope methods; Immunofluorescence; Infectious pancreatic necrosis virus (IPNV); SYBR-Green I.
Resumo: | A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.14 n.1 2011