Data/hora: 06/06/2024 06:32:07

Provedor de dados: | 72
País: | Chile
Título: | AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
Autores: | Aguilera-Muñoz,Felipe; Valenzuela-Muñoz,Valentina; Gallardo-Escárate,Cristian.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | Genetic traceability; ITS 1; ITS2; Mo Husk identification; Specific divider design.
Resumo: | Contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. In this context, Chile has reached an important development in agricultural exportations, reaching near US$ 3.82 billion in 2007. Since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. This study analyzed five species of Chilean commercial mo Husks using molecular DNA markers. From the partial sequences of the ribosomal region ITSl-5.8SrDNA-ITS2, the specific primers for the internal transcribed spacers 1 and 2 (ITS1, ITS2) were designed. These primers in 8 fresh species of mollusks, 5 commercial presentations and 3 processed species, obtaining specie-specific band patterns in all cases for both ITS. Additionally, the primers are shown to be highly specific and of replicable amplification products, independently from the commercial presentation of the studied species. Genetically trace aquaculture products. The presence of fraud for species substitution between canned abalone (Haliotis) and loco (Concholepas) species is discussed.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000200007
Editor: | Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
Formato: | text/html
Fonte: | Gayana (Concepción)  v.72 n.2 2008