Data/hora: 07/06/2024 10:25:23

Provedor de dados: | 74
País: | Brazil
Título: | Developmental and hyperthermia-induced expression of the heat shock proteins HSP60 and HSP70 in tissues of the housefly Musca domestica: an in vitro study
Autores: | Sharma,Sunita; Rohilla,Manoj Singh; Tiwari,Pramod Kumar.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Heat stress response; HSP60; HSP70; Musca domestica; Thermoprotection; Housefly.
Resumo: | The expression pattern of two major chaperones, the heat shock proteins (HSPs) HSP60 and HSP70 was studied in vitro in tissues of the housefly Musca domestica during larval and adult stages of development to identify their immunological relatives and understand their functional significance in normal cellular activities and during thermal stress. Fluorographs of labeled polypeptides and western blots demonstrated that both HSPs are expressed constitutively and heat-induced in all the larval and adult cell types examined. The pattern of whole tissue immunocytochemical staining using anti-HSP60 and anti-HSP70 antibodies corresponded well with the observations from western blots or fluorographs. In developing oocytes, both constitutive and heat inducible expression of HSP60 were regulated in an oocyte stage-specific manner. In unstressed ovaries the expression of these proteins was less pronounced in early stage oocytes (1st - 8th) than at later stages (9th and onward). The heat shock, however, induced both HSP70 and HSP60 to a significantly high level in early stage oocytes (1st-8th) as compared to their respective controls. Our findings indicate the involvement of the HSP60 and HSP70 proteins in the development, growth and differentiation of both somatic and germ line tissues. Furthermore, the enhanced co-expression of HSP70 and HSP60 upon heat shock in various larval and adult cell types suggests the possible role of HSP60 in thermoprotection.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100027
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000100027
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess