Data/hora: 14/06/2024 00:49:11

Provedor de dados: | 74
País: | Brazil
Título: | Identification of proliferating cells in chicken embryos using 5-bromo- 2'-deoxyuridine immunohistochemical detection
Autores: | Lopes,Mário Lúcio; Schmidt,Gilberto Silber; Coutinho,Luiz Lehmann.
Data: | 2000-03-01
Ano: | 2000
Resumo: | Chicken embryos were incubated with BrdU, embedded in plastic resin, sectioned and screened immunohistochemically to identify proliferating cells in the neural tube and somites. Fixation in 4% paraformaldehyde for 1 h was essential for detecting specific colorimetric signals of BrdU incorporation into cells during the S phase of the cell cycle. Transverse sections of the neural tube showed that the nuclei of proliferating cells (BrdU positive) had a uniform and centralized distribution, whereas unstained nuclei were found only along the extremities of the neural tube. Transverse sections of differentiated somites showed proliferating cells in the scleratome and dermatome. However, no incorporation of BrdU was observed in myotomic cells, which give rise to axial skeletal muscle. In spite of their proximity, the dermatome and myotome showed marked differences in cell proliferation. The excellent preservation of morphological characteristics in the embryonic tissues facilitated identification of variations in BrdU incorporation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100028
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572000000100028
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.23 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess