Data/hora: 12/06/2024 01:43:12

Provedor de dados: | 74
País: | Brazil
Título: | Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)
Autores: | Bombonato,Juliana Rodrigues; Bonatelli,Isabel Aparecida Silva; Silva,Gislaine Angélica Rodrigues; Moraes,Evandro Marsola; Zappi,Daniela Cristina; Taylor,Nigel P.; Franco,Fernando Faria.
Data: | 2019-03-01
Ano: | 2019
Palavras-chave: | Cactaceae; Cereus; Cross-genera; SSR markers; Transferability.
Resumo: | Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2017-0293
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.42 n.1 2019
Fonte: | info:eu-repo/semantics/openAccess