Data/hora: 06/06/2024 01:17:15

Provedor de dados: | 74
País: | Brazil
Título: | Identification of new isolates of Bacillus thuringiensis using rep-PCR products and delta-endotoxin electron microscopy
Autores: | Lima,A.S.G.; Guidelli,A.M.; Abreu,I.L.; Lemos,M.V.F.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | REP; ERIC; Crystal protein; Parasporal bodies.
Resumo: | PCR has been used to analyze the distribution of REP (Repetitive Extragenic Palindromic) and ERIC (Enterobacterial Repetitive Intergenic Consensus) sequences (rep-PCR) found within the genome of the bacterium Bacillus thuringiensis, with the purpose to analyze the genetic similarities among 56 subspecies samples and 95 field isolates. The PCR products were analyzed by EB-AGE (ethidium bromide-agarose electrophoresis) and then submitted to banding comparisons, based on the Phyllip software algorithm. When the banding similarities were considered for comparison purposes among all the strains, the phylogenic tree patterns varied according to the rep-PCR primers considered, but, from a broader point of view, the ERIC sequences produced better results, which, together with electron microscopy analysis of the released parasporal bodies and colony morphology characteristics, allowed to detect two possible new subspecies of B. thuringiensis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200017
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572002000200017
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.25 n.2 2002
Fonte: | info:eu-repo/semantics/openAccess