Data/hora: 01/06/2024 07:38:10

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain	
Autores: 	Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui.	
Data: 	2014-06-01	
Ano: 	2014	
Palavras-chave: 	Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization.	
Resumo: 	Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization exhibited the CHY-like reactivity presented in resorptive cells of the hepatopancreas. After bacterial challenge with Vibrio alginolyticus, the expression level of SpCHY mRNA was extremely up-regulated at 3 h in hepatopancreas. Our results suggest that SpCHY might play an important role in the mud crab's immune response.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572014005000007	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.37 n.2 2014	
Fonte: 	info:eu-repo/semantics/openAccess