Data/hora: 06/06/2024 11:26:01

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Analysis of castor bean ribosome-inactivating proteins and their gene expression during seed development	
Autores: 	Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs.	
Resumo: 	Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most expressed RIPs in developing seeds although eight other RIPs were also expressed. All of the RIP genes were most highly expressed in the stages in which the endosperm was fully expanded. Although the reason for the large expansion of RIP genes in castor beans remains to be established, the differential expression patterns of the type I and type II members reinforce the existence of biological functions other than defense against predators and herbivory.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572013005000005	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.36 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess