Data/hora: 11/06/2024 12:44:02

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila	
Autores: 	Mota,Adolfo Jose; Back-Brito,Graziella Nuernberg; Nobrega,Francisco G.	
Data: 	2012-01-01	
Ano: 	2012	
Palavras-chave: 	Candida palmioleophila; Debaryomyces hansenii; Differential identification; Pichia guilliermondii; Real-time PCR.	
Resumo: 	Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100017	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572011005000059	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.35 n.1 2012	
Fonte: 	info:eu-repo/semantics/openAccess