Data/hora: 13/06/2024 06:27:45

Provedor de dados: | 74
País: | Brazil
Título: | Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis
Autores: | Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira.
Data: | 2005-09-01
Ano: | 2005
Palavras-chave: | Genetic variability; Bacterium; Citrus; PFGE.
Resumo: | Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the strains. Plasmids were observed in all strains, with a total of five different plasmids, with sizes between 57.7 and 83.0 kilobases. The 72.6 kb plasmid was the most frequent, present in 15 out of 22 strains, while the 68.1 kb plasmid was observed in two strains only. Although the plasmid diversity detected in the present study was not very great, the X. axonopodis pv. citri strains evaluated showed a considerable degree of diversity with regard to this extrachromosomal genetic element.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000300020
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.3 2005
Fonte: | info:eu-repo/semantics/openAccess