Data/hora: 09/06/2024 14:52:25

Provedor de dados: | 74
País: | Brazil
Título: | Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
Autores: | Chaves,Paulo B.; Paes,Marcela F.; Mendes,Sérgio L.; Strier,Karen B.; Louro,Iúri D.; Fagundes,Valéria.
Data: | 2006-01-01
Ano: | 2006
Palavras-chave: | Brachyteles; Conservation genetics; Endangered species; Fecal DNA; Noninvasive sampling.
Resumo: | The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400028
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572006000400028
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.29 n.4 2006
Fonte: | info:eu-repo/semantics/openAccess