Data/hora: 01/06/2024 08:59:04

Provedor de dados: | 74
País: | Brazil
Título: | Sucrose synthase molecular marker associated with sugar content in elite sugarcane progeny
Autores: | Silva,Jorge A. da; Bressiani,Jose A.
Data: | 2005-01-01
Ano: | 2005
Palavras-chave: | Sucrose; Synthase; Expressed; Sequence; Tag.
Resumo: | We describe the development and application of an expressed sequence tag (EST)-derived restriction fragment length polymorphism (RFLP) marker for sugarcane elite genotypes which can be used for quantitative trait loci (QTL) tagging for sugar content. EST-derived RFLP markers for proteins involved in sucrose metabolism have been used in Southern analysis for mapping and gene tagging in elite sugarcane clones. A single dose marker, obtained from a sucrose synthase EST associated with sugar content at the alpha = 0.01 probability level, is presented for sugarcane breeding. Utilization of EST homologues to known genes for generation of molecular markers accelerated the identification of a QTL controlling an important trait-sugar content. Sugarcane bacterial artificial chromosome (BAC) clones hybridizing to the sucrose synthase EST were identified.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200020
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572005000200020
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.28 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess