Data/hora: 02/06/2024 04:53:08

Provedor de dados: | 74
País: | Brazil
Título: | A leukocyte cryopreservation technique for cytogenetic studies
Autores: | Duarte,José Maurício Barbanti; Ramalho,Mariney Flávia Pereira Di Tano; Lima,Vera Fernanda Hossepian de; Jorge,Wilham.
Data: | 1999-09-01
Ano: | 1999
Resumo: | Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300019
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47571999000300019
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.22 n.3 1999
Fonte: | info:eu-repo/semantics/openAccess