Data/hora: 05/06/2024 08:10:01

Provedor de dados: 	74	
País: 	Brazil	
Título: 	A leukocyte cryopreservation technique for cytogenetic studies	
Autores: 	Duarte,José Maurício Barbanti; Ramalho,Mariney Flávia Pereira Di Tano; Lima,Vera Fernanda Hossepian de; Jorge,Wilham.	
Data: 	1999-09-01	
Ano: 	1999	
Resumo: 	Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinez (VYM) solution with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP) as cryopreservatives. The best protocol for freezing was 4°C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still frozen material was placed onto cultivation medium for propagation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000300019	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47571999000300019	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.22 n.3 1999	
Fonte: 	info:eu-repo/semantics/openAccess