Data/hora: 01/06/2024 01:04:00

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Preferential induction of MLL (Mixed Lineage Leukemia) rearrangements in human lymphocyte cultures treated with etoposide	
Autores: 	Brassesco,María Sol; Montaldi,Ana Paula; Sakamoto-Hojo,Elza Tiemi.	
Data: 	2009-01-01	
Ano: 	2009	
Palavras-chave: 	Etoposide; FISH; MLL translocations.	
Resumo: 	Topoisomerase II inhibitors are effective chemotherapeutic agents in the treatment of cancer, in spite of being associated with the development of secondary leukemia. Our purpose was to determine the effects of etoposide on different genomic regions, aiming at discovering whether there are preferential sites which can be targeted by this drug in peripheral lymphocytes from healthy individuals. The in vitro treatment with low doses of etoposide (0.25, 0.5, and 1 µg/mL, in 1 hour-pulse or continuous-48 h treatment) induced a significant increase in chromosomal aberrations, detected by conventional staining and FISH with specific probes for chromosomes 8 and 11, compared with untreated controls (p &lt; 0.05). Additionally, the frequencies of alterations at 11q23, detected by MLL specific probes, were significantly higher (p &lt; 0.005) in treated cells than in controls. In contrast, an analysis of rearrangements involving the IGH gene did not disclose differences between treatments. The present results demonstrated the potential of etoposide to interact with preferential chromosome sites in human lymphocytes, even at concentrations below the mean plasma levels measured in cancer patients. This greater susceptibility to etoposide-induced cleavage may explain the more frequent involvement of MLL in treatment-related leukemia.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100022	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572009000100022	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.32 n.1 2009	
Fonte: 	info:eu-repo/semantics/openAccess