Data/hora: 10/06/2024 15:17:15

Provedor de dados: | 74
País: | Brazil
Título: | Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
Autores: | Almeida,Terezinha M.B. de; Leitão,Regina Maria C.; Carrilho,Flair J.; Sonohara,Shigueko.
Data: | 2010-01-01
Ano: | 2010
Palavras-chave: | Micronuclei; Hepatocytes; Chronic hepatitis C; Fibrosis progression; HCV-patients.
Resumo: | Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fastgreen staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p &lt; 0.05) and higher than those in the control group. Like results were also obtained on comparing F4 with F0, F1, F2 and F3 cases. Conversely, differences were not significant (p &amp;gt; 0.05) on comparing F0, F1, F2, F3, one against the other, as well as individual versus control. Although chromosomal losses in CH were detected, it was shown that liver parenchyma with fibrosis in the initial stages (F1-F3) cannot be considered cytogenetically abnormal.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300003
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572010005000061
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.33 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess