Data/hora: 17/06/2024 03:59:25

Provedor de dados: | 74
País: | Brazil
Título: | Identification and detection of a novel human endogenous retrovirus-related gene, and structural characterization of its related elements
Autores: | Liang,Qiaoyi; Ding,Jiayi; Zheng,Shu.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Endogenous retrovirus; Noncoding RNA; RT-PCR; Quantitative real-time PCR; Transcription.
Resumo: | Up-regulation of human endogenous retroviruses (HERVs) is associated with many diseases, including cancer. In this study, an H family HERV (HERV-H)-related gene was identified and characterized. Its spliced transcript lacks protein-coding capacity and may belong to the emerging class of noncoding RNAs (ncRNAs). The 1.3-kb RNA consisting of four exons is transcribed from an Alu element upstream of a 5.0-kb structurally incomplete HERV-H element. RT-PCR and quantitative RT-PCR results indicated that expression of this HERV-related transcript was negatively associated with colon, stomach, and kidney cancers. Its expression was induced upon treatment with DNA methylation and histone deacetylation inhibitors. A BLAT search using long terminal repeats (LTRs) identified 50 other LTR homogenous HERV-H elements. Further analysis of these elements revealed that all are structurally incomplete and only five exert transcriptional activity. The results presented here recommend further investigation into a potentially functional HERV-H-related ncRNA.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400005
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009005000082
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess