Data/hora: 17/06/2024 15:28:32

Provedor de dados: | 74
País: | Brazil
Título: | Expression profiles of sugarcane under drought conditions: Variation in gene regulation
Autores: | Andrade,Júlio César Farias de; Terto,Jackeline; Silva,José Vieira; Almeida,Cícero.
Data: | 2015-12-01
Ano: | 2015
Palavras-chave: | Gene expression; Plant breeding; Quantitative RT-PCR.
Resumo: | Abstract Drought is a major factor in decreased sugarcane productivity because of the resulting morphophysiological effects that it causes. Gene expression studies that have examined the influence of water stress in sugarcane have yielded divergent results, indicating the absence of a fixed pattern of changes in gene expression. In this work, we investigated the expression profiles of 12 genes in the leaves of a drought-tolerant genotype (RB72910) of sugarcane and compared the results with those of other studies. The genotype was subjected to 80–100% water availability (control condition) and 0–20% water availability (simulated drought). To analyze the physiological status, the SPAD index, Fv/Fm ratio, net photosynthesis (A), stomatal conductance (gs) and stomatal transpiration (E) were measured. Total RNA was extracted from leaves and the expression of SAMDC, ZmPIP2-1 protein, ZmTIP4-2 protein, WIP protein, LTP protein, histone H3, DNAj, ferredoxin I, β-tubulin, photosystem I, gene 1 and gene 2 was analyzed by quantitative real-time PCR (RT-PCR). Important differences in the expression profiles of these genes were observed when compared with other genotypes, suggesting that complex defense mechanisms are activated in response to water stress. However, there was no recognizable pattern for the changes in expression of the different proteins associated with tolerance to drought stress.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400465
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738420140288
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.4 2015
Fonte: | info:eu-repo/semantics/openAccess