Data/hora: 06/06/2024 09:42:59

Provedor de dados: | 74
País: | Brazil
Título: | Soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes): isolated isoforms and kinetics properties
Autores: | Aquino-Silva,Maria Regina de; Schwantes,Maria Luiza Barcellos; Munin,Flavia Simone; Schwantes,Arno Rudi; Santos,Silvana Pereira dos.
Data: | 2008-01-01
Ano: | 2008
Palavras-chave: | Gene duplication; SMDH; Substrate concentration; Temperature; PH isoforms.
Resumo: | Kinetic properties and thermal stabilities of Geophagus brasiliensis skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to examine a possible sMDH-B* locus duplication in a fixation process influenced by genetic drift. Two optimal pHs were detected: 7.5 for AB1 unfractionated muscle phenotype and its B1 isoform, and 8.0 for AB1B2 unfractionated muscle phenotype, A and B2 isoforms. While G. brasiliensis A isoform could be characterized as thermostable, the duplicated B isoform cannot be assumed as thermolabile. Km values for isolated B2 isoforms were 1.6 times lower than for B1. A duplication event in progress best explains the electrophoretic six-band pattern detected in G. brasiliensis, which would be caused by genetic drift.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200029
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572008000200029
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.31 n.1 suppl.0 2008
Fonte: | info:eu-repo/semantics/openAccess