Data/hora: 06/06/2024 08:12:59

Provedor de dados: | 74
País: | Brazil
Título: | Simultaneous overexpression of GH and STAT5b genes inhibits the STAT5 signalling pathway in tilapia (Oreochromis niloticus) embryos
Autores: | Marins,Luis Fernando; Iyengar,Arati; Maclean,Norman; Levy,Jose A.; Sohm,Frédéric.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Teleost; Growth hormone; Signal transduction; Cytokine; Trangenesis signal transducers and activators of transcription.
Resumo: | In this study, we describe the use of a STAT5 responsive element (LHRE) reporter gene to monitor the activity of the growth hormone (GH) transduction pathway following expression of heterologous fish GH and rat STAT5b in tilapia embryos and fish fibroblast cells. Our results indicate that both GH and STAT5b are able to activate the LHRE at high levels when transferred separately, demonstrating the substantial level of conservation of the GH signal transduction pathways between fish and mammals. Unexpectedly, co-expression experiments show a strong inhibition of the GH-dependent activation, suggesting that simultaneous GH and STAT5b overexpression can counteract effects of GH expression in tilapia embryos.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300007
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572002000300007
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.25 n.3 2002
Fonte: | info:eu-repo/semantics/openAccess