Data/hora: 09/06/2024 14:44:38

Provedor de dados: | 74
País: | Brazil
Título: | Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation
Autores: | Diniz,Michely C.; Costa,Marcília P.; Pacheco,Ana C.L.; Kamimura,Michel T.; Silva,Samara C.; Carneiro,Laura D.G.; Sousa,Ana P.L.; Soares,Carlos E.A.; Souza,Celeste S.F.; Oliveira,Diana Magalhães de.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Actin-interacting proteins (AIPs); Flagellar proteins; Leishmania; Coronin and Arp2/3; Phagosome.
Resumo: | Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid virulence. In a search for AIPs involved in flagellar activities, we applied computational biology and proteomic tools to infer from the biological meaning of coronins and Arp2/3, two important elements in phagosome formation after parasite phagocytosis by macrophages. Results presented here provide the first report of Leishmania coronin and Arp2/3 as flagellar proteins that also might be involved in phagosome formation through actin polymerization within the flagellar environment. This is an issue worthy of further in vitro examination that remains now as a direct, positive bioinformatics-derived inference to be presented.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300033
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009000300033
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess