Data/hora: 01/06/2024 16:27:38

Provedor de dados: | 74
País: | Brazil
Título: | Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system
Autores: | Junqueira,Adriana Pereira Freire; Perazzo,Fabio Ferreira; Souza,Gustavo Henrique Bianco; Maistro,Edson Luis.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Micronucleus test; Piper cubeba; Single cell gel electrophoresis (SCGE).
Resumo: | The plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg-1, 1.0 g kg-1 and 1.5 g kg-1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g kg-1, the highest dose tested, the extract induced a statistically significant increase in both the mean number of micronucleated polychromatic erythrocytes and the level of DNA damage in the rodent cell types analyzed. Under our experimental conditions, the P. cubeba seed extract was genotoxic in vivo when administered orally to mice and rats.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400025
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572007000400025
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.30 n.3 2007
Fonte: | info:eu-repo/semantics/openAccess