Data/hora: 01/06/2024 03:33:26

Provedor de dados: | 74
País: | Brazil
Título: | Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Autores: | Du,Yue-feng; Liang,Liang; Shi,Ying; Long,Qing-zhi; Zeng,Jin; Wang,Xin-yang; He,Da-lin.
Data: | 2012-01-01
Ano: | 2012
Palavras-chave: | Prostate cancer; DNA methylation; DNMT3; RNA interference.
Resumo: | Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100024
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572012005000021
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.35 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess