Data/hora: 01/06/2024 03:34:03

Provedor de dados: | 74
País: | Brazil
Título: | Genome composition and pollen viability of Jatropha (Euphorbiaceae) interspecific hybrids by Genomic In Situ Hybridization (GISH)
Autores: | Souza,Rosilda Cintra de; Marques,Daniela de Argollo; de Carvalho Filho,Marcel Mamede; Oliveira,Ana Rafaela da Silva; Siqueira,Walter José; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Interspecific crosses; Jatropha integerrima; J. multifida; Physic nut; Plant breeding.
Resumo: | Abstract Interspecific hybridization is required for the development of Jatropha curcas L. improved cultivars, due to its narrow genetic basis. The present study aimed to analyze the parental genomic composition of F1 and BC1F1 generations derived from interspecific crosses (J. curcas/J. integerrima and J. curcas/J. multifida) by GISH (Genomic In Situ Hybridization), and the meiotic index and pollen viability of F1 hybrids. In F1 cells from both hybrids, 11 chromosomes of each parental was observed, as expected, but chromosome rearrangement events could be detected using rDNA chromosome markers, suggesting unbalanced cells. In the BC1F1, both hybrids had 22 chromosomes, suggesting that only n = 11 gametes were viable in the next generation. However, GISH allowed the identification of three and two alien chromosomes in J. curcas//J. integerrima and J. curcas//J. multifida BC1F1 hybrids, respectively, suggesting a preferential transmission of J. curcas chromosomes for both hybrids. Pollen viability in F1 hybrids derived from J. curcas/J. integerrima crosses were higher (82-83%) than those found for J. curcas/J. multifida (68%), showing post-meiotic problems in these last hybrids, with dyads, triads, polyads, and micronuclei as post-meiosis results. The here presented cytogenetic characterization of interspecific hybrids and their backcross progenies can contribute to the selection of the best genotypes for future assisted breeding of J. curcas.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500302
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2019-0112
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.42 n.4 2019
Fonte: | info:eu-repo/semantics/openAccess