Data/hora: 11/06/2024 19:01:44

Provedor de dados: | 74
País: | Brazil
Título: | RAPD markers linked to a block of genes conferring rust resistance to the common bean
Autores: | Faleiro,Fábio Gelape; Vinhadelli,Wender Santos; Ragagnin,Vilmar Antonio; Corrêa,Ronan Xavier; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
Data: | 2000-06-01
Ano: | 2000
Resumo: | Rust, caused by the fungus Uromyces appendiculatus, may cause a significant loss to common bean (Phaseolus vulgaris L.) yield. RAPD markers tightly linked to the resistance genes may be used in breeding programs to aid the development of rust-resistant bean cultivars. In this sense, the objective of the present work was to identify RAPD markers linked to a rust resistance gene block present in the cultivar Ouro Negro. Two hundred and fourteen F2 individuals from a cross between the resistant cultivar Ouro Negro and the susceptible cultivar US Pinto 111 were inoculated with a mixture of eight races of U. appendiculatus. The segregation ratio obtained suggested that resistance is monogenic and dominant. Bulked segregant analysis was used in conjunction with the RAPD technique to search for markers linked to rust resistance genes. Two molecular markers flanking the rust resistance gene block were identified, one at 5.8 ± 1.6 cM (OX11(630)) and the other at 7.7 ± 1.7 cM (OF10(1,050)) of the gene. Simulated indirect selection efficiency in the F2 population using the two markers was 100%. The molecular markers identified in this work are currently being used for the selection of disease-resistant plants in the commom bean breeding program of the Federal University of Viçosa.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200027
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572000000200027
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.23 n.2 2000
Fonte: | info:eu-repo/semantics/openAccess