Data/hora: 11/06/2024 04:49:38

Provedor de dados: 	74	
País: 	Brazil	
Título: 	Identification of alternative transcripts of rat CD9 expressed by tumorigenic neural cell lines and in normal tissues	
Autores: 	Wolfahrt,Sonja; Herman,Sandra; Scholz,Claus-Jürgen; Sauer,Georg; Deissler,Helmut.	
Data: 	2013-01-01	
Ano: 	2013	
Palavras-chave: 	CD9; Tetraspanin; Transcript; Splice variant; Membrane topology.	
Resumo: 	CD9 is the best-studied member of the tetraspanin family of transmembrane proteins. It is involved in various fundamental cellular processes and its altered expression is a characteristic of malignant cells of different origins. Despite numerous investigations confirming its fundamental role, the heterogeneity of CD9 or other tetraspanin proteins was considered only to be caused by posttranslational modification, rather than alternative splicing. Here we describe the first identification of CD9 transcript variants expressed by cell lines derived from fetal rat brain cells. Variant mRNA-B lacks a potential translation initiation codon in the alternative exon 1 and seems to be characteristic of the tumorigenic BT cell lines. In contrast, variant mRNA-C can be translated from a functional initiation codon located in its extended exon 2, and substantial amounts of this form detected in various tissues suggest a contribution to CD9 functions. From the alternative sequence of variant C, a different membrane topology (5 transmembrane domains) and a deviating spectrum of functions can be expected.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200019	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572013000200019	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.36 n.2 2013	
Fonte: 	info:eu-repo/semantics/openAccess